کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1212789 | 1494041 | 2016 | 8 صفحه PDF | دانلود رایگان |
• We developed a LC–MS/MS method to quantify oxidized monoesters of DEHTP in urine.
• Target analytes were chromatographically separated from DEHP metabolite analogues.
• Urine samples of the general population were positively tested for target analytes.
• Oxidized monoesters are promising biomarkers of exposure for human biomonitoring.
Di(2-ethylhexyl) terephthalate (DEHTP) is used as a substitute for ortho-phthalate based plasticizers like di(2-ethylhexyl) phthalate (DEHP) which are discussed and regulated due to their reproductive toxicity. We developed a fast and rugged method to quantify side chain oxidized monoesters of DEHTP in human urine, namely 5OH-MEHTP, 5oxo-MEHTP, 2cx-MMHTP and 5cx-MEPTP. Sample preparation was kept simple with enzymatic deconjugation and a two column assembly for on-line sample clean up. Metabolites were identified with authentic standards and quantified via isotope dilution LC–MS/MS. The limit of quantification was 0.2 μg/L for 5cx-MEPTP and 5oxo-MEHTP, 0.3 μg/L for 5OH-MEHTP and 0.4 μg/L for 2cx-MMHTP. Accuracy (relative recovery: 95.8–111%) and precision (relative standard deviation: <7%) were highly acceptable. In a pilot biomonitoring study with 34 volunteers (aged 25–61 (median 42), 20 female and 14 male) not known to be occupationally exposed to DEHTP, we could detect 5cx-MEPTP above the limit of quantification in 94% of the samples (median: 0.9 μg/L, maximum: 38.7 μg/L). The other metabolites investigated were detected at a lower rate and at lower concentration levels (5oxo-MEHTP: 21%, maximum: 1.8 μg/L; 5OH-MEHTP: 18%, maximum: 3.4 μg/L; 2cx-MMHTP: 9%, maximum: 0.9 μg/L). All target analytes can be regarded as promising and specific urinary biomarkers for DEHTP exposure. With this method we provide a basis for quantitatively investigating the human metabolism of DEHTP and for performing exposure and risk assessments in the general population and the working environment.
Journal: Journal of Chromatography B - Volume 1011, 1 February 2016, Pages 196–203