کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1216365 967009 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Validation of HPLC analysis method of a novel antihypertensive agent MS23 in rat plasma
چکیده انگلیسی

MS23 is a vasodilator with unique dual action pharmacological profile to inhibit type 4 PDE and antagonize L-type calcium channels. We validated an analytical protocol for MS23 in rat plasma using high performance liquid chromatography (HPLC). A C18 column and a phosphate/acetonitrite buffer were used for chromatographic separation. UV detection was performed at 307 nm. The calibration curve for MS23 was linear in the range from 50 to 10,000 ng/ml. The limit of quantification (LOQ) was 50 ng/ml. The results demonstrate that the method has linearity (R = 0.9989), specificity, and acceptable precision/accuracy. This method is simple, economic, and sufficient for in vivo pharmacokinetic studies on the compound.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 830, Issue 1, 2 January 2006, Pages 13–17
نویسندگان
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