کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1220245 | 967771 | 2011 | 8 صفحه PDF | دانلود رایگان |
The phenolic profile and anticancer properties of extracts from whole cowpeas, seed coats and cotyledons were determined. Seed coats contained at least 5 and 10 times more free and bound phenolics compared to whole seeds (75.6 and 31.7 mg/100 g of free and bound phenolics, respectively). Seed coats and cotyledons contained about 50% and 95% of free phenolics, respectively. The major phenolics associated to seed coats were gallic and protocatechuic acid, whereas in cotyledons p-hydroxybenzoic acid was prevalent. Seed coats contained approximately 10 times more flavonoids compared to whole seeds and cotyledons were practically free of flavonoids. After acid hydrolysis, myricetin, quercetin and kaempferol were identified in seed coats. Most of the antioxidant activity determined with the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assays was exerted by free phenolics. The free phenolic extract of whole seeds at a concentration of approximately 100 mg gallic acid equiv. (GAE)/l inhibited 65% the proliferation of hormone-dependent mammary (MCF-7) cancer cells. Extracts of seed coats or cotyledons also inhibited cell proliferation but to a significantly lesser extent, thus indicating synergistic effects between phenolics and other phytochemicals associated to these anatomical parts.
► This paper describes for the first time free and bound phenolics in the different anatomical parts of cowpeas.
► Details the antioxidant capacity by ORAC and ATPS assays of free and bound phenolics of cowpeas.
► Confirms the presence of specific phenolic acids found in cowpeas and their distribution in its anatomical parts.
► Demonstrate the anticancer effects of free phytochemicals of whole cowpeas against mammary cancer cells (MCF-7).
Journal: Journal of Functional Foods - Volume 3, Issue 4, October 2011, Pages 290–297