کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1225672 968242 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The development of a peptide SRM-based tandem mass spectrometry assay for prenatal screening of Down syndrome
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The development of a peptide SRM-based tandem mass spectrometry assay for prenatal screening of Down syndrome
چکیده انگلیسی

Two new biomarkers, serum amyloid-P (SAP) and plasma C1-inhibitor protein are elevated in the maternal circulation of mothers carrying Down syndrome foetuses. Much emphasis of late\ has been put on the lack of translational tests being developed following the identification of new biomarkers. We have created a single-reaction-monitoring (SRM) tandem mass spectrometry-based assay for the quantitation of these biomarkers and compared these results with an in-house developed immunofluorescence-based technique (IF). This MS-based assay is a rapid 5 min test and a simple “one pot reaction,” requiring only 5 μl of plasma. To evaluate the potential of SRM-based quantitation in a clinical setting, SAP and C1-inhibitor were quantitated in 38 normal and Down syndrome affected pregnancies. Plasma SAP levels in the Down's group were significantly raised at 10–14 weeks (p < 0.0015) and 14–20 weeks (p < 0.0001). Plasma C1-inhibitor levels were also observed significantly elevated in the Down's group (10–14 weeks, p < 0.0193, 14–20 weeks, p < 0.0001). Analysis using the IF technique did not show any significant elevation of plasma SAP levels or C1-inhibitor levels. This rapid and sensitive assay demonstrates the potential of multiplexed tandem MS-based quantitation of proteins in chemical pathology labs and in a more cost-effective, accurate manner than conventionally used antibody methods.

Figure optionsDownload high-quality image (26 K)Download as PowerPoint slideHighlights
► We have developed a novel method to quantitate two new plasma markers for Down syndrome screening.
► Unique peptide single reaction monitoring assays were set up.
► Complete quantitation of both proteins was achieved in 5 min with 5 μl plasma.
► The new markers were evaluated against, gestational age, foetal sex and maternal weight.
► We have shown that the assay is an improvement on conventional immunofluorescence tests.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Proteomics - Volume 75, Issue 11, 18 June 2012, Pages 3248–3257
نویسندگان
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