کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1226652 | 968316 | 2014 | 5 صفحه PDF | دانلود رایگان |
It is not clear how frequent is copper deficiency in humans. Current copper markers are not sensitive enough to detect early copper deficiency and new markers are needed. CCS is a candidate to become a copper biomarker.ObjectiveMeasuring CCS mRNA relative expression in malnourished children and compare results (a) with those of the same children after nutritional recovery and (b) with well-nourished children.MethodOn admission to the protocol and after 15 day nutritional treatment, severely (G1 = 18) and moderately (G2 = 10) malnourished children were compared with well-nourished healthy controls (G3 = 15), measuring anthropometric indicators, blood biochemistry, Cu, Fe and Zn serum concentrations, ceruloplasmin, C Reactive protein and mRNA abundance of CCS, SOD and MT2 in peripheral mononuclear cells.ResultIn malnourished groups, mean serum copper concentration was below the cut-off on admission to hospital and increased after 15 days (t-test, p < 0.01). On admission to protocol, CCS mRNA abundance in G1 and G2 was higher than in G3 (one way ANOVA, p < 0.001). After 15 days, CCS expression decreased as expected (t-test, p < 0.001). Initial SOD mRNA relative abundance was higher in study groups than controls and also between G1 and G2 (One way ANOVA, both p < 0.01); after 15 days, G1 and G2 were not different (t-test, NS). MT2A abundance of transcripts did not follow a clear change pattern.ConclusionCCS mRNA abundance responded as expected, being higher in malnourished children than in controls; nutritional recovery in these latter resulted in decreasing expression of the chaperone, supporting the hypothesis that CCS may be a copper biomarker.
Journal: Journal of Trace Elements in Medicine and Biology - Volume 28, Issue 1, January 2014, Pages 23–27