A symmetrical subtraction combined with interpolated values for eliminating scattering from fluorescence EEM data

• A symmetrical subtraction method for eliminating scattering from EEM is proposed.
• Symmetrical subtraction combined with interpolated values was discussed.
• Nine methods were used for comparison.
• A suitable method depends on interested components and their environment.
• Both combination of results and methods can be tried to obtain a good result.

Parallel factor analysis is a widely used method to extract qualitative and quantitative information of the analyte of interest from fluorescence emission-excitation matrix containing unknown components. Big amplitude of scattering will influence the results of parallel factor analysis. Many methods of eliminating scattering have been proposed. Each of these methods has its advantages and disadvantages. The combination of symmetrical subtraction and interpolated values has been discussed. The combination refers to both the combination of results and the combination of methods. Nine methods were used for comparison. The results show the combination of results can make a better concentration prediction for all the components.

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