کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1228995 | 1495224 | 2015 | 7 صفحه PDF | دانلود رایگان |
• Binding ability of a new pyranochromene derivative (3-HC) with DNA has been evaluated.
• Cytotoxic activity of 3-HC was analyzed against K562 cell line.
• The compound possesses significant cytotoxic and radical scavenging properties.
The binding properties of a new pyranochromene derivative, 2-amino-4-(3-hydroxyphenyl)-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (3-HC) with calf thymus DNA (ctDNA) have been investigated by UV–vis absorption, circular dichroism, fluorescence spectroscopy and viscosity measurement. These results indicated that 3-HC can interact with DNA through non-intercalative mode and the intrinsic binding constant (Kb) for 3-HC with DNA was estimated to be 3.6 × 103 M−1. The antioxidant activity experiments show that 3-HC also exhibit good antioxidant activity in DPPH free radical scavenging and ferric reducing ability methods. Moreover, 3-HC exhibited cytotoxic activity against K562, human chronic myelogenous leukemia cells, with IC50 value of 146 μM and the cells responded to the treatment with mostly through apoptosis.
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Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 145, 15 June 2015, Pages 353–359