Optimization of β-cyclodextrin cross-linked polymer for monitoring of quercetin

• The investigation of the inclusion interactions of β-CDCP with quercetin.
• The adsorption behavior of Qu on β-CDCP.
• The β-CDCP could be used repeatedly for 30 times.
• This technique had been successfully applied to the determination of Qu in real samples.

A novel method for the separation/analysis of quercetin was described, which was based on the investigation of the inclusion interactions of β-cyclodextrin cross-linked polymer (β-CDCP) with quercetin (Qu) and the adsorption behavior of Qu on β-CDCP. The inclusion interaction of β-CDCP with Qu was studied through FTIR, TGA and 13C NMR. Under the optimum conditions, the preconcentration factor of the proposed method was approximately 8.8, the β-CDCP could be used repeatedly for 30 times and offered better recovery. The linear range, limit of detection (LOD) and the relative standard deviation (RSD) was found to be 0.10–12.0 μg mL−1, 4.6 ng mL−1 and 3.10% (n = 3, c = 2.0 μg mL−1) respectively. This technique had been successfully applied to the determination of Qu in real samples.

There were some obvious changes of characteristic absorption peaks for the β-CDCP−Qu: (1) σCC peak (1612 cm−1) in β-CDCP−Qu vanished; the width of peak at 3390 cm−1 was larger in β-CDCP−Qu; which was demonstrated that the aromatic part in Qu was included into the hydrophobic cavity of β-CDCP. (2) The intensity of σCOH peaks at 1333 cm−1 and 1244 cm−1 in β-CDCP−Qu were much higher than that of β-CDCP, it was probably attributed to the fact that the hydrogen bond interactions played a crucial role in the inclusion of β-CDCP−Qu.Figure optionsDownload as PowerPoint slide