Study of the interaction of salmon sperm DNA with myricitrin–CPB based on the enhanced resonance light scattering signal and its potential application

• A new method determining DNA concentration was proposed.
• The linear range was 0.076–4.2 μg mL−1.
• The detection limit was 4.1 ng mL−1.

A new assay of salmon sperm DNA at nanogram level was established based on enhanced resonance light scattering (RLS) signals of DNA with myricitrin and cetylpyridinium bromide dihydrate (CPB). The RLS spectral characteristics of DNA with myricitrin–CPB and the optimum conditions for determination of DNA samples have been studied. At pH 7.4, myricitrin–CPB could enhance the intensity of RLS signal of DNA at 468 nm. The enhanced RLS intensity was directly proportional to DNA concentration in the range of 0.076–4.2 μg mL−1 with a good linear relationship (r = 0.9944). The detection limit was 4.1 ng mL−1. The synthetic samples were analyzed with satisfactory results that the recovery was 100.9–102.6% and RSD was 1.4–2.1%, which proved that the new method was reliable and applicable.

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