کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1238399 | 1495286 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resonance light scattering technique for the determination of proteins with polymethacrylic acid (PMAA)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Resonance light scattering technique for the determination of proteins with polymethacrylic acid (PMAA) Resonance light scattering technique for the determination of proteins with polymethacrylic acid (PMAA)](/preview/png/1238399.png)
چکیده انگلیسی
As a resonance light scattering (RLS) probe, the polyelectrolyte polymethacrylic acid (PMAA) was applied in this assay. The bovine serum albumin (BSA) and human serum albumin (HSA) were determined by the electrostatic interaction of PMAA and proteins. At pH 3.8 Na2HPO4-citric acid buffer solution, the RLS intensities of PMAA-BSA (HSA) system were greatly enhanced. The characteristic peaks were appeared at the wavelength 320, 546 and 594Â nm. The optimization conditions of the reaction were also examined and selected. Under the selected conditions, the RLS intensities were proportional to the protein concentrations in the range of (0.0200-2.00)Â ÃÂ 10â6Â mol/L for BSA and (0.0200-2.40)Â ÃÂ 10â6Â mol/L for HSA. The influences of some foreign substances were also examined. The synthetic samples containing proteins and some real samples were analyzed and the results obtained were satisfactory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 67, Issues 3â4, July 2007, Pages 1126-1130
Journal: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy - Volume 67, Issues 3â4, July 2007, Pages 1126-1130
نویسندگان
Yanhua Chen, Dejiang Gao, Yuan Tian, Peng Ai, Hanqi Zhang, Aimin Yu,