Detection of HIT antibody dependent platelet aggregation using novel surface imprinting approach

• This is the first report of platelet imprinting.
• HIT platelet aggregation can be measured by using this approach.
• Surface MIP based QCM-D enables monitoring of platelet aggregation directly in PRP thus eliminating the challenge of platelet separation.
• QCM-D technology can be applied for functional measurements of HIT antibodies.

We present a fast, robust and straightforward spin force assisted surface imprinting approach for activated platelets and demonstrate that Heparin induced thrombocytopenia (HIT) platelet aggregation can be measured by this approach. A critical and challenging step in functional assays for HIT is platelet separation from the healthy donor's platelet-rich plasma (PRP). Our approach using surface imprinted polymer (MIP) for measurements on a quartz crystal microbalance with dissipation (QCM-D) enables monitoring of platelet aggregation directly in PRP thus eliminating the challenge of platelet separation. This is the first report of platelet imprinting. We also provide proof of principle that QCM-D technology can be applied for functional measurements of HIT antibodies.

We present a fast, robust and straightforward spin force assisted surface imprinting approach for activated platelets and demonstrate that Heparin induced thrombocytopenia (HIT) platelet aggregation can be measured by this approach. A critical and challenging step in functional assays for HIT is platelet separation from the healthy donor's platelet-rich plasma (PRP). Our approach using surface imprinted polymer (MIP, “plastic antibodies”) for measurements on a quartz crystal microbalance with dissipation (QCM-D) enables monitoring of platelet aggregation directly in PRP thus eliminating the challenge of platelet separation. This is the first report of platelet imprinting. We also provide proof of principle that QCM-D technology can be applied for functional measurements of HIT antibodies. With this report we demonstrate a novel spin force assisted surface imprinting approach for activated platelets and its applicability for measurements of HIT antibody induced platelet aggregation. The imprinting process is depicted below (Scheme 1).Figure optionsDownload as PowerPoint slide