کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1247714 | 1379093 | 2016 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deoxyribonucleic acid glycosylase assays: Progress and prospects
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Deoxyribonucleic acid (DNA) plays an extremely important role in storing genetic messages that are used for cellular functions in living organisms. However, the process of replicating these messages is not free from errors as internal and external factors can contribute to the damages of DNA strands. These damages known as lesions are problematic since they can lead to mutagenesis and carcinogenesis after accumulation for a certain period of time. Thankfully, cells are able to synthesize various DNA glycosylases to target different lesions, repairing and restoring damaged DNA to its original state. This review article aims to explore the variety of assays used to evaluate the efficiency of these glycosylases, mainly focusing on uracil DNA glycosylase, thymine DNA glycosylase, and 8-oxoguanine DNA glycosylase since they are deployed to the sites of damages much more often than other repair enzymes. The progress in the development of DNA glycosylase assays is reviewed with an emphasis on the configuration and operating mechanism of the assays and with some discussion on challenges and prospects. It is hoped that this article will provide an introductory overview and the current standing of DNA glycosylase assays, opening up new perspectives towards the development DNA glycosylase assays for uses in molecular biology and at point-of-care.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 83, Part B, October 2016, Pages 102-115
Journal: TrAC Trends in Analytical Chemistry - Volume 83, Part B, October 2016, Pages 102-115
نویسندگان
Hua Zheng Ng, Melanie Ng, Cher Mui Eng, Zhiqiang Gao,