کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1248140 | 1495931 | 2012 | 12 صفحه PDF | دانلود رایگان |
Time-resolved luminescence has been employed as a method of detection for electrophoretic studies for nearly two decades. We discuss experiments conducted on capillary-zone electrophoresis, capillary-gel electrophoresis, slab-gel electrophoresis and microchip electrophoresis. We place emphasis on the variety of sample molecules, and applications in order to show the versatility of this technique.
► Electrophoresis experiments using time-resolved luminescence detection are reviewed.
► Solutions to theory, experiment, and instrument-based challenges are discussed.
► Non-lifetime modes of detection can reduce background and improve sensitivity.
► Lifetime modes of detection permit peak identification and multiplexing by lifetime.
► More relevant methods need to be developed using time-resolved detection.
Journal: TrAC Trends in Analytical Chemistry - Volume 33, March 2012, Pages 23–34