کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1254372 1379131 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sequence identification, structure prediction and validation of tannase from Aspergillusniger N5-5
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Sequence identification, structure prediction and validation of tannase from Aspergillusniger N5-5
چکیده انگلیسی

Tannases produced by filamentous fungi are in a family of important hydrolases of gallotannins and have broad industry applications. But until now, the 3-D structures of fungi tannases have not been reported. The protein sequence deduced from the cDNA sequence obtained using RT-PCR amplification was identified as tannase through sequence alignment and phylogenetic analysis. Structure models based on the tannase sequence were collected using I-TASSER, and the model with the best match to the surface charge density-pH titration profile was selected as the final structure for tannase from Aspergillusniger N5-5. This work provides an effective method for protein structure research. The structure constructed in this work should be very important to understand the enzyme bioactivities and further developments of fungi tannases.

The protein sequence deduced from the cloned tannase gene sequence from Aspergillus niger N5-5 was identified as tannase through sequence alignment and phylogenetic analysis. Then, the enzyme 3-D structure was predicted using I-TASSER and experimentally validated.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Chemical Letters - Volume 27, Issue 7, July 2016, Pages 1087–1090
نویسندگان
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