A selective and sensitive off–on probe for palladium and its application for living cell imaging

A new rhodamine B derivative T1 has been rationally synthesized and displayed selective Pd(II)-amplified absorbance and fluorescence emission above 540 nm in methanol–water. Upon the addition of Pd(II), the spirolactam ring was unfolded and a 1:1 metal-ligand complex formed, which can be used for “naked-eyes” detection. In addition, fluorescence imaging experiments of Pd2+ in HepG2 living cells showed its valuable application in biological systems.

A new rhodamine B “off–on” Probe T1 has been rationally synthesized and displayed selective Pd(II)-amplified absorbance and fluorescence emission in methanol–water. Upon the addition of Pd(II), the spirolactam ring was unfold and a 1:1 metal-ligand was formed, which can be used for “naked-eye” detection and living cells fluorescence imaging.Figure optionsDownload as PowerPoint slide