کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1268898 | 972427 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Steady-state bioluminescence of bacterial luciferase using electrochemical regeneration of flavin substrate and its application to inhibitory analysis
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The model system for the biological reaction using a bacterial luciferase (BL) was developed and applied to the inhibitory analysis of the hydrophobic molecules for enzymatic reactions. The homemade flow electrochemical luminescence cell was embedded in the BL reaction system to regenerate the reduced form of the flavin mononucleotide, which is one of the substrates of the BL luminescence reaction, and to measure the luminescence intensity. The constant intensity of the continuous BL luminescence was observed using the continuous-flow BL reaction system. The proposed system was successfully applied to the inhibitory reaction of dodecaneamide on the BL luminescence reaction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 75, Issue 1, April 2009, Pages 67–70
Journal: Bioelectrochemistry - Volume 75, Issue 1, April 2009, Pages 67–70
نویسندگان
Shinya Yamasaki, Shinya Nakashima, Shuto Yamada, Kô Takehara,