کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1377898 | 981990 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A dye-labelled soluble substrate for the assay of endo-chitosanase activity
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
Coupling Remazol Brilliant Blue R (RBB) to chitosan that has been subjected to partial enzymatic hydrolysis gave rise to a new dyed derivative of chitosan which is highly soluble in slightly acidic buffers or microbiological media. The RBB:chitosan ratio has been carefully optimized, as well as the reaction conditions, to obtain a quasi-linear, sensitive assay for chitosanase. The substrate (sRBB-C) is useful for microtitre plate-based screening and for assay of chitosanase activity in the presence of high backgrounds of reducing sugars. Due to its solubility, sRBB-C is particularly suitable for the detection of chitosanase-positive organisms on agar media.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 80, Issue 2, 12 April 2010, Pages 521–524
Journal: Carbohydrate Polymers - Volume 80, Issue 2, 12 April 2010, Pages 521–524
نویسندگان
Mina Zitouni, Mélanie Fortin, Jean-Sébastien Thibeault, Ryszard Brzezinski,