Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620

• The LTA has been isolated from allergy-protective Staphylococcus sciuri W620 strain.
• Its structure has been elucidated utilizing NMR spectroscopy.
• Structural analyses included hydrophilic backbone and glycolipid anchor.

Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria. LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments. Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids. The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.

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