کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1384593 1500603 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The heparin-like activities of negatively charged derivatives of low-molecular-weight polymannuronate and polyguluronate
ترجمه فارسی عنوان
فعالیت-هپارین مانند مشتقات بار منفی از polymannuronate مولکولی کم وزن و polyguluronate
کلمات کلیدی
Polymannuronate؛ Polyguluronate؛ مشتقات پلی آنیون؛ Heparinoid؛ ضد انعقاد؛ FGFs / سیگنالینگ FGFR1c
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی


• LPM and LPG were prepared from alginate and studied side-by-side.
• LPM and LPG derivatives containing −PO32−, −PO2H− and −SO3− were synthesized.
• SO3− modification of LPM and LPG enhanced the anticoagulant and FGFs/FGFR1c signaling activation activities.
• Sulfated LPG had better heparin-like activities than sulfated LPM.

A series of low-molecular-weight polymannuronate (LPM) and polyguluronate (LPG) polyanionic derivatives, including LPM/LPG phosphate (LPMP/LPGP), LPM/LPG H-phosphonate (LPMHP/LPGHP) and LPM/LPG sulfate (LPMS/LPGS), were prepared as heparinoids by chemical modification of LPM and LPG. The structures and characteristics of LPM, LPG and their derivatives were elucidated based on high performance gel permeation chromatography (HPGPC), fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and polyacrylamide gel electrophoresis (PAGE). In order to test the heparin-like activities of these derivatives and to reveal the activities affected by substituent groups and PM/PG polysaccharide backbones, the anticoagulant activities and FGF/FGFR1c signaling activation abilities were evaluated in vitro. The results showed that sulfate group was the best substituent group to improve the heparin-like activities of LPM/LPG compared with the other two anionic groups. The results also showed that sulfated derivative based on PG structure had better activities than that based on PM structure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 155, 2 January 2017, Pages 313–320
نویسندگان
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