Structural elucidation of fungal polysaccharides isolated from the cell wall of Plectosphaerella cucumerina and Verticillium spp.

The structure of acidic fungal polysaccharides isolated from the cell wall of Plectosphaerella cucumerina, Verticillium dahliae, and V. albo-atrum has been investigated by chemical analysis, methylation analysis, and 1D and 2D 1H and 13C NMR spectroscopy. The polysaccharides have an idealized repeating block of the type: linked to a small mannan core (<15%), where n = 13, m = 13, p = 5, and q = 8 for P. cucumerina, and n = 16, m = 16, p = 6, and q <1 for both Verticillium species.

The idealized repeating unit of the polysaccharide isolated from P. cucumerina is proposed to be:Figure optionsDownload as PowerPoint slide