کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1393655 | 983966 | 2014 | 7 صفحه PDF | دانلود رایگان |
• We identify a distinct mechanism for aryl-aldehyde generation in polyketides
• An NRPS-like protein can activate and reduce an aryl-acid to an aryl-aldehyde
• Such a distinct mechanism may be widely used throughout the fungi kingdom
SummaryAryl-aldehydes are a common feature in fungal polyketides, which are considered to be exclusively generated by the R domain of nonreducing polyketide synthases (NR-PKSs). However, by cloning and heterologous expression of both cryptic NR-PKS and nonribosomal peptide synthase (NRPS)-like genes from Aspergillus terreus in Saccharomyces cerevisiae, we identified a distinct mechanism for aryl-aldehyde formation in which a NRPS-like protein activates and reduces an aryl-acid produced by the accompanying NR-PKS to an aryl-aldehyde. Bioinformatics study indicates that such a mechanism may be widely used throughout the fungi kingdom.
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Journal: - Volume 21, Issue 2, 20 February 2014, Pages 257–263