Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-Aryl-substituted 2-bis-1H-benzimidazoles

Inhibitors of topoisomerase I constitute a novel family of antitumor agents. The class of benzimidazole derivatives contains compounds possessing affinity to DNA. For example, fluorescent stains Hoechst 33342 and Hoechst 33258 interact with DNA as ligand and produce nonspecific inhibition of the catalytic activity of many enzymes involved in DNA synthesis, including DNA topoisomerase and DNA helicase. Several 2-aryl-5-substituted-2,5-bisbenzimidazole derivatives were synthesized and ability of these derivatives to induce DNA cleavage in the presence of topoisomerase I was evaluated in vitro. These analogs were also assayed for their cytotoxicity against U87, MCF7 and HeLa human tumor cells. All the four compounds showed a potent growth inhibitory effect on all the cell lines, with IC50 in the μM range.

A series of 2-bisbenzimidazole derivatives were synthesized and evaluated as topoisomerase I inhibitors. Compound 6b was found to be the most potent inhibitor.Figure optionsDownload as PowerPoint slideResearch highlights
► The manuscript describes the synthesis and characterization of new analogues of Hoechst 33342 having bi and tri substitution on phenyl ring.
►  The manuscript evaluates the cytotoxic effect of five bisbenzimdazoles on U87, MCF7 and HeLa cell lines.
► Topoisomerase I inhibition assay was performed to determine the target of these molecules.