Water-insoluble glucans from the edible fungus Ramaria botrytis

Two water-insoluble glucans were isolated from the fruiting bodies of an edible fungus, Ramaria botrytis by extracting with sodium hydroxide. Structural characterizations of these glucans were investigated on the basis of total hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments (1H, 13C, TOCSY, DQF-COSY, and HSQC) and the structures of the repeating units were established as: