A high sensitive electrochemical nanosensor for simultaneous determination of glutathione, NADH and folic acid

• Electrooxidation of glutathione was studied by novel modified nanocomposite electrode.
• The sensor resolved the overlap response of glutathione, NADH and folic acid.
• The new sensor was used for the determination of above compounds in some real samples.
• Electrosynthesis of a new catechol derivative as a novel mediator was studied.

In the present study, we report electrosynthesis of 4,5-bis(4-chloroanilino)-1,2-benzendiol (BCB) and its application as a selective electrochemical mediator at a surface of carbon paste electrode (CPE) modified ZnO/CNTs nanocomposite as a simple and rapid voltammetric sensor. The sensor showed an efficient catalytic activity for the electro-oxidation of glutathione (GSH), which leads to a lowered overpotential by more than 203 mV compared to unmodified carbon paste electrode. For the mixture containing GSH, nicotinamide adenine dinucleotide (NADH) and folic acid (FA), the electrooxidation signals were well separated. The square wave voltammetry (SWV) currents increased linearly with their concentration at the ranges of 0.006–161, 1.0–650 and 3.0–700 μM, respectively with the detection limits of 0.002, 0.3 and 1.0 μM. Finally, the electrode was successfully applied for the voltammetric determination of analytes in real samples with satisfactory results.

In this study, carbon-paste electrode modified with a new electrosynthesized catechol derivative, 4,5-bis(4-chloroanilino)-1,2-benzendiol, as a novel mediator and ZnO/CNTs was used as a suitable sensor for simultaneous voltammetric determination of glutathione, NADH and folic acid for the first time.Figure optionsDownload as PowerPoint slide