کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429392 987174 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of the protocol used for fibroin extraction on the mechanical properties and fiber sizes of electrospun silk mats
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Influence of the protocol used for fibroin extraction on the mechanical properties and fiber sizes of electrospun silk mats
چکیده انگلیسی

Silk fibroin (SF) was regenerated using three of the most common protocols described in the bibliography for the dissolution of raw SF (LiBr 9.3 M, CaCl2 50 wt.% or CaCl2:EtOH:H2O 1:2:8 in molar ratio). The integrity of regenerated SF in aqueous solution was analyzed by SDS-PAGE and different profiles of degradation were observed depending on the protocol used. This fact was found to affect also the aqueous solubility of the freeze dried protein. These different SFs were used to produce electrospun mats using SF solutions of SF 17 wt.% in 1,1,1,1′,1′,1′-hexafluoro-2-propanol (HFIP) and significant differences in fiber sizes, elongation and ultimate strength values were found. This work provides a global overview of the manner that different methods of SF extraction can affect the properties of electrospun SF-mats and consequently it should be considered depending on the use they are going to be made for.

Figure optionsDownload as PowerPoint slideHighlights
► Protocol employed to dissolve raw SF affects diameter of electrospun fibers.
► Mw of SF polypeptides plays an important role in the fiber sizes of the mats.
► The highest aqueous solubility of lyophilized SF is when it is dissolved in LiBr 9.3 M.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 33, Issue 4, 1 May 2013, Pages 1945–1950
نویسندگان
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