کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1487588 | 1510711 | 2015 | 7 صفحه PDF | دانلود رایگان |
• Ni(OH)2/HAP nanorods are synthesized by hydrothermal method.
• Their morphology depends strongly on the reaction condition.
• Ni(OH)2/HAP nanorods can be used to separate His-tagged proteins.
• Ni(OH)2/HAP nanorods present high protein binding ability.
Nickel hydroxide/hydroxyapatite (designated as Ni(OH)2/HAP) nanorods have been designed and synthesized by hydrothermal method. The size and morphology of Ni(OH)2/HAP sample depend strongly on the reaction condition, and introduction of small amount of Ni2+ ions would be propitious to get the smaller size nanorods. By taking advantage of the high affinity of Ni2+ ions toward histidine-tagged (His-tagged) proteins, the Ni(OH)2/HAP nanorods can be used to enrich and separate His-tagged proteins directly from a mixture of lysed cells, and their universality was evaluated by separating three kinds of His-tagged proteins. The Ni(OH)2/HAP nanorods present negligible nonspecific protein adsorption and high protein binding ability, and their specificity and affinity toward His-tagged proteins can be remained after 4 times recycling. The Ni(OH)2/HAP nanorods may provide many potential applications for the detection or separation of different biomolecules.
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Journal: Materials Research Bulletin - Volume 64, April 2015, Pages 200–206