کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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149053 | 456426 | 2013 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Chitosan–halloysite hybrid-nanotubes: Horseradish peroxidase immobilization and applications in phenol removal Chitosan–halloysite hybrid-nanotubes: Horseradish peroxidase immobilization and applications in phenol removal](/preview/png/149053.png)
Chitosan–halloysite hybrid-nanotubes were synthesized through the assembly of chitosan onto halloysite, a natural nanotubular aluminosilicate. Nitrogen adsorption–desorption measurement, Fourier-transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy analysis were employed to elucidate the structure of the hybrid-nanotubes. The results indicated that the hybrid-nanotubes could form three-dimensional (3D) nanocomposites with hierarchically porous structure. As-prepared structure showed excellent capacity for horseradish peroxidase (HRP) immobilization through cross-linking by glutaraldehyde. The maximum enzyme loading reached as large as 21.5 mg/g, higher than 3.1 mg/g of raw halloysite. After 35 days storage, the immobilized HRP did not undergo any activity loss while the free HRP only retained 27% of its original activity. Phenol removal efficiency by the immobilized HRP was also explored. The result showed the immobilized HRP exhibited overall high removal efficiency for phenol from wastewater.
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► Assembling chitosan onto the halloysite to form hybrid-nanotubes.
► High enzyme loading through covalent binding method.
► About 100% retention rate of original activity for immobilized horseradish peroxidase.
► High removal efficiency for phenol by immobilized enzyme.
Journal: Chemical Engineering Journal - Volume 214, 1 January 2013, Pages 304–309