کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
155488 | 456897 | 2013 | 11 صفحه PDF | دانلود رایگان |
A model-based rational protein purification process synthesis methodology that addresses the challenge of selecting the most optimal process scheme from several possible alternatives is presented in this study. The main rationale behind the methodology is to keep the number of purification units in the final process to the minimum through a systematic cycle of flowsheet synthesis, optimization, evaluation and the rational elimination of the least feasible process options at each purification step, taking into account the specific needs of the purification step. Process evaluation is based on techno-economic performance obtained by model-based optimization of the integrated processes using validated column models. The methodology was illustrated by synthesizing a process for the purification of monoclonal antibody from crude hybridoma cell culture supernatant using four non-affinity chromatographic methods (AEX, CEX, SEC and HIC). The results showed that four out of thirteen evaluated process options satisfied all pre-defined product specifications, with overall yields ≥90%. In order of increasing product cost, these were CEX–AEX
► Optimization of integrated process flowsheets using validated column models.
► The number of purification units in the final process is kept to the minimum.
► Purification cascades are evaluated under their optimized working conditions.
► The process synthesis methodology is generic for cell-derived proteins.
► The methodology was illustrated by a case study.
Journal: Chemical Engineering Science - Volume 89, 15 February 2013, Pages 185–195