Investigation of air-DBD effects on biological liquids for in vitro studies on eukaryotic cells

Atmospheric pressure (AP) Dielectric Barrier Discharges (DBD) generated in air were performed on water, Phosphate Buffer Saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) in order to evaluate their potential to generate hydrogen peroxide (H2O2), nitrite (NO2-) and nitrate (NO3-) species. Differences in cell growth and morphology were found in two different types of eukaryotic cells, immortal and stem cells, incubated for 1 h in plasma treated DMEM, revealing a selectivity of the treatment.