کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1762509 | 1019698 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Encapsulation of Concentrated Protein Into Erythrocyte Porated by Continuous-Wave Ultrasound
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موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
آکوستیک و فرا صوت
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چکیده انگلیسی
A procedure of continuous-wave ultrasound (US)-induced hemolysis and reseal in solution containing water soluble protein was applied to a method for encapsulating concentrated protein solutions into resealed rat erythrocyte ghosts. To find a condition yielding a higher mean corpuscular concentration of encapsulated protein (MCC), we investigated the value of MCCs for various conditions. Additions of a small amount of plasma, Ca2+ and Mg2+ significantly increased MCC, whereas these additives did not alter the degree of hemolysis. It was suggested that plasma protect the molecular damages by the US, and that Ca2+ and Mg2+ physically stabilized the lipids of the erythrocyte membrane to fuse and reseal the pore induced by US. A maximal MCC of â¼50 mg/mL, which is 2.5 times the reported maximum amount encapsulated by the osmotic dialysis method, was obtained without a blood-washing procedure. (E-mail: masaaki.iino@it-chiba.ac.jp)
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasound in Medicine & Biology - Volume 34, Issue 12, December 2008, Pages 1924-1933
Journal: Ultrasound in Medicine & Biology - Volume 34, Issue 12, December 2008, Pages 1924-1933
نویسندگان
Koutarou Yamagata, Emi Kawasaki, Hideki Kawarai, Masaaki Iino,