کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
176548 | 458946 | 2013 | 8 صفحه PDF | دانلود رایگان |
• New NIR cationic dyes based on benzo[a]phenoxazinium chlorides were synthesised.
• The dyes showed intercalative behaviour, aggregation or groove binding in DNA.
• They display promising capabilities as DNA coloured markers in electrophoresis assays.
Cationic dyes based on benzo[a]phenoxazinium chlorides either mono- or disubstituted with ethyl, octyl and dodecyl groups at the amino side chain at the 9-position of the tetracyclic ring, and possessing a propyl group at the 5-amino position were synthesised, characterised and used in photophysical studies with DNA and in agarose gel electrophoresis assays. Photophysical results showed that compounds with diethyl and octyl substituents intercalated in DNA above a phosphate dye ratio of 10. All compounds associated with DNA as evidenced by their use as coloured markers of migrating DNA bands in agarose gel electrophoresis experiments.
A new set of NIR fluorescent benzo[a]phenoxazinium chlorides was synthesised. Photophysical studies and agarose gel electrophoresis experiments with these Nile Blue derivatives revealed their potential as DNA markers and probes.Figure optionsDownload as PowerPoint slide
Journal: Dyes and Pigments - Volume 99, Issue 1, October 2013, Pages 220–227