کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
177854 459004 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A study of the binding of C.I. Direct Yellow 9 to human serum albumin using optical spectroscopy and molecular modeling
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A study of the binding of C.I. Direct Yellow 9 to human serum albumin using optical spectroscopy and molecular modeling
چکیده انگلیسی

The mechanism of interaction between C.I. Direct Yellow 9 and human serum albumin was studied using spectroscopic methods including fluorescence spectra, UV–vis, Fourier transform infrared (FT-IR) and circular dichroism (CD). The quenching mechanism was investigated in terms of the association constants, number of binding sites and basic thermodynamic parameters. The distance between the human serum albumin donor and the acceptor dye was 3.64 nm as derived from fluorescence resonance energy transfer. Alteration of the secondary protein structure in the presence of the dye was confirmed by UV, FT-IR and CD spectroscopy. Molecular modeling revealed that a dye–protein complex was stabilized by hydrophobic forces and hydrogen bonding, via amino acid residues.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 79, Issue 2, November 2008, Pages 176–182
نویسندگان
, , , ,