کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
188898 459669 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor
چکیده انگلیسی

Glucose oxidase (GOx) was stably immobilized via a simple physical adsorption method onto the nanostructured Au thin films fabricated by using electrodeposition and galvanic replacement technology, which provides a facile method to prepare morphology-controllable Au films and also facilitates the preparation and application of enzyme modified electrodes. An obvious advantage of the as-prepared enzyme electrode (denoted as GOx/Au/GCE) is that the nano-Au films provide a favorable microenvironment for GOx and facilitate the electron transfer between the active center of GOx and electrodes. Cyclic voltammetry (CV) results indicate that the immobilized GOx displayed a direct, reversible and surface-confined redox reaction in the phosphate buffer solution. Furthermore, the enzyme modified electrode was used as a glucose bioelectrochemical sensor, exhibiting a linear relationship in the concentration ranges of 2.5–32.5 μmol L−1 and 60–130 μmol L−1 with a detection limit of 0.32 μmol L−1 (S/N = 3) at an applied potential of −0.55 V. Due to the excellent stability, sensitivity and anti-interference ability, the Au thin films are hopeful in the construction of glucose biosensors.

Figure optionsDownload as PowerPoint slideHighlights
► Au thin films are formed by electrodeposition and galvanic replacement technology.
► Glucose oxidase is stably immobilized via a simple physical adsorption method.
► The direct electrochemical behavior is obtained on the immobilized glucose oxidase.
► An amperometric sensor of glucose with an excellent sensing capability is achieved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 67, 15 April 2012, Pages 140–146
نویسندگان
, , , , ,