کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1927814 1050261 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Contact-dependent abrogation of bone marrow-derived plasmacytoid dendritic cell differentiation by murine mesenchymal stem cells
ترجمه فارسی عنوان
انقباض وابسته به تماس جداسازی سلول دندریتیک پلاسمایی مشتق از مغز استخوان توسط سلول های بنيادی مزانشيمی موش صحرايی
کلمات کلیدی
سلولهای دندریتیک؛ گیرنده Toll مانند؛ اینترفرون-a؛ سلولهای استرومای مزانشیمی؛ التهاب
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Mesenchymal stem cells (MSC) suppress plasmacytoid dendritic cell (pDC) generation.
• MSC-mediated inhibition of pDC differentiation is contact-dependent.
• MSC-mediated inhibition is pDC-specific as myeloid DC generation is not suppressed.

Plasmacytoid dendritic cells (pDCs) are rare central regulators of antiviral immunity and unsurpassed producers of interferon-α (IFN-α). Despite their crucial role as a link between innate and adaptive immunity, little is known about the modulation of pDC differentiation by other bone marrow (BM) cells. In this study, we investigated the modulation of pDC differentiation in Flt-3 ligand (Flt3L)-supplemented BM cultures, using highly purified mesenchymal stem cells (MSCs) that were FACS-isolated from murine BM based on surface marker expression and used after in vitro expansion. Initial analysis revealed an almost complete inhibition of BM-derived pDC expansion in the presence of >2% MSC. This inhibition was cell contact-dependent and soluble factor-independent, as indicated by trans-well experiments. The abrogation of functional pDC development by MSCs was confirmed after TLR9 stimulation, revealing a complete, contact-dependent suppression of the IFN-a producing capacity of pDCs in Flt3L MSC BM co-cultures. MSC selectively inhibited pDC development in contrast to myeloid DC development, as indicated by the significantly increased numbers of myeloid DC in Flt3L-supplemented BM cultures. The absence of significant MSC-mediated inhibitory effects on myeloid DC differentiation was confirmed by additional experiments in GM-CSF/IL-4-supplemented BM cultures. In summary, we describe a novel contact-dependent immunomodulatory mechanism of MSC that targets the BM-derived expansion of functional pDCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 476, Issue 1, 15 July 2016, Pages 15–20
نویسندگان
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