The role of C-terminal amino acid residues of a Δ6-fatty acid desaturase from blackcurrant

► The C-terminal 67 aa of blackcurrant D6D is essential for its catalytic activity. ► Of the 67 aa, six site mutations lead to a significant decrease in D6D activity. ► Of the 67 aa, one aa site mutation leads to a significant increase in D6D activity. ► N, T & Y in the last four aa of the C-terminal play some role in D6D activity.