Characterization and comparison of protein complexes initiated by the intracellular domain of individual Notch paralogs

► Assembly of Notch intracellular domain (NICD)-containing protein complexes is dose-dependent. ► MAML becomes the limiting factor for formation of canonical NICD complex when NICD level is high. ► There are differences in the NICD protein complexes formed by individual Notch paralogs. ► Among the four NICDs, Notch4 ICD shows a unique protein complex profile.