کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1935020 | 1050656 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Physcomitrella HMGA-type proteins display structural differences compared to their higher plant counterparts
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کلمات کلیدی
GFPTCAHmgAT-hook - AT-قلابElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزDNA-binding - اتصال DNAtrichloroacetic acid - اسید ترشکلراکتیکGene expression - بیان ژنTranscription - رونویسیEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکsubcellular localisation - محلی سازی سلولgreen fluorescent protein - پروتئین فلورسنت سبزChromatin - کروماتینhigh mobility group - گروه تحرک بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
High mobility group (HMG) proteins of the HMGA family are chromatin-associated proteins that act as architectural factors in nucleoprotein structures involved in gene transcription. To date, HMGA-type proteins have been studied in various higher plant species, but not in lower plants. We have identified two HMGA-type proteins, HMGA1 and HMGA2, encoded in the genome of the moss model Physcomitrella patens. Compared to higher plant HMGA proteins, the two Physcomitrella proteins display some structural differences. Thus, the moss HMGA proteins have six (rather than four) AT-hook DNA-binding motifs and their N-terminal domain lacks similarity to linker histone H1. HMGA2 is expressed in moss protonema and it localises to the cell nucleus. Typical of HMGA proteins, HMGA2 interacts preferentially with A/T-rich DNA, when compared with G/C-rich DNA. In cotransformation assays in Physcomitrella protoplasts, HMGA2 stimulated reporter gene expression. In summary, our data show that functional HMGA-type proteins occur in Physcomitrella.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 374, Issue 4, 3 October 2008, Pages 653-657
Journal: Biochemical and Biophysical Research Communications - Volume 374, Issue 4, 3 October 2008, Pages 653-657
نویسندگان
Carina Lyngaard, Christian Stemmer, Allan Stensballe, Manuela Graf, Gilbert Gorr, Eva Decker, Klaus D. Grasser,