کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1938119 | 1050732 | 2007 | 7 صفحه PDF | دانلود رایگان |
The retinoic acid related orphan receptor RORα activates transcription of genes that play an important role in cerebellar development, the protection against age-related degenerative processes, the regulation of inflammatory responses, and is one of the pivotal participants that control the circadian rhythmicity in the core-clock of mammals. We identified the extracellular signal-regulated kinase 2 (ERK-2) as RORα4 phosphorylating kinase in vitro. The primary sequence of RORα4 contains an ERK-2 recognition motif (P-L-T128-P) within the hinge domain, and mutation of Thr-128 to Ala prevents RORα4 phosphorylation by ERK. The RORα4-T128A mutant exhibits an increased DNA-binding affinity, an increased transcriptional activity and, in the interplay with the opponent RevErbα, acts as a stronger competitor at ROR response elements than RORα4-WT.
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 3, 6 July 2007, Pages 890–896