کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941706 1536903 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Possible activation by the green tea amino acid theanine of mammalian target of rapamycin signaling in undifferentiated neural progenitor cells in vitro
ترجمه فارسی عنوان
فعال سازی ممکن توسط تینین اسید آمینه اسید چای سبز از هدف پستانداران سیگنال گیری رپامایسین در سلول های پیش گیاه شناس عصبی در آزمایشگاهی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Theanine promotes mTOR phosphorylation in neural progenitors.
• Theanine promotes the formation of embryonic and adult neurospheres.
• SLC38A1 overexpression promotes mTOR phosphorylation in pluripotent P19 cells.
• Theanine fails to further promote mTOR phosphorylation in SLC38A1 transfectants.
• Theanine promotes subsequent neuronal differentiation in embryonic progenitors.

We have shown marked promotion of both proliferation and neuronal differentiation in pluripotent P19 cells exposed to the green tea amino acid theanine, which is a good substrate for SLC38A1 responsible for glutamine transport. In this study, we evaluated the activity of the mammalian target of rapamycin (mTOR) kinase pathway, which participates in protein translation, cell growth and autophagy in a manner relevant to intracellular glutamine levels, in murine neural progenitor cells exposed to theanine. Exposure to theanine promoted the phosphorylation of mTOR and downstream proteins in neurospheres from embryonic mouse neocortex. Although stable overexpression of SLC38A1 similarly facilitated phosphorylation of mTOR-relevant proteins in undifferentiated P19 cells, theanine failed to additionally accelerate the increased phosphorylation in these stable transfectants. Theanine accelerated the formation of neurospheres from murine embryonic neocortex and adult hippocampus, along with facilitation of both 5-bromo-2’-deoxyuridine incorporation and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide reduction in embryonic neurospheres. In embryonic neurospheres previously exposed to theanine, a significant increase was seen in the number of cells immunoreactive for a neuronal marker protein after spontaneous differentiation. These results suggest that theanine activates the mTOR signaling pathway for proliferation together with accelerated neurogenesis in murine undifferentiated neural progenitor cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 5, March 2016, Pages 89–95
نویسندگان
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