کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941714 1536903 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Macrophage depletion by clodronate liposome attenuates muscle injury and inflammation following exhaustive exercise
ترجمه فارسی عنوان
تخلیه ماکروفاژ توسط لیپوزوم کلودرونات کاهش آسیب عضلانی و التهاب پس از ورزش کامل
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Exhaustive exercise enhanced macrophage infiltration in skeletal muscle tissue
• Clodronate liposome induced macrophage depletion in skeletal muscle after exercise
• Macrophage depletion decreased skeletal muscle myofiber injury and cytokine levels
• Macrophages increased muscle injury by regulating inflammation

Exhaustive exercise promotes muscle injury, including myofiber lesions; however, its exact mechanism has not yet been elucidated. In this study, we tested the hypothesis that macrophage depletion by pretreatment with clodronate liposomes alters muscle injury and inflammation following exhaustive exercise. Male C57BL/6J mice were divided into four groups: rest plus control liposome (n=8), rest plus clodronate liposome (n=8), exhaustive exercise plus control liposome (n=8), and exhaustive exercise plus clodronate liposome (n=8). Mice were treated with clodronate liposome or control liposome for 48 h before undergoing exhaustive exercise on a treadmill. Twenty-four hours after exhaustive exercise, the gastrocnemius muscles were removed for histological and PCR analyses. Exhaustive exercise increased the number of macrophages in the muscle; however, clodronate liposome treatment reduced this infiltration. Although exhaustive exercise resulted in an increase in injured myofibers, clodronate liposome treatment following exhaustive exercise reduced the injured myofibers. Clodronate liposome treatment also decreased the mRNA expression levels of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in the skeletal muscle after exhaustive exercise. These results suggest that macrophages play a critical role in increasing muscle injury by regulating inflammation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 5, March 2016, Pages 146–151
نویسندگان
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