کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1964243 | 1058536 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
βγ subunits of Gi/o suppress EGF-induced ERK5 phosphorylation, whereas ERK1/2 phosphorylation is enhanced
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کلمات کلیدی
myocyte enhancer factorPC12GRKEpacLPACTXPTXEGFRTKGPCRG3PDHTRISNGFGPCR kinasepKaRASDMEMERKIGFRT-PCRReceptor tyrosine kinase (RTK)Pertussis toxin (PTX)HRPMEFBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز G-protein-coupled receptor - G-پروتئین گیرندهG-protein-coupled receptor (GPCR) - G-پروتئین گیرنده (GPCR)MAPK - MAPKDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoTris-buffered saline containing 0.1% Tween-20 - Tris-buffered saline حاوی 0.1٪ Tween-20 استlysophosphatidic acid - اسید لیسفسفیدیدlysophosphatidic acid (LPA) - اسید لیسفسفیدید (LPA)RTK, Receptor tyrosine kinase - تیروزین کینازهای گیرنده ایPC12 cells - سلول های PC12pheochromocytoma cells - سلولهای فئوکروموسیتوماpertussis toxin - سموم سورافنیcholera toxin - سوزش گوارشیEpidermal growth factor (EGF) - عامل رشد اپیدرمال (EGF)epidermal growth factor - عامل رشد اپیدرمیnerve growth factor - فاکتور رشد عصبNerve growth factor (NGF) - فاکتور رشد عصبی (NGF)Insulin-like growth factor - فاکتور رشد مانند انسولینBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزLAD - لادوreverse transcription–polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسHorseradish peroxidase - پراکسیداز هوررادیشexchange protein activated by cAMP - پروتئین مبادله فعال شده توسط cAMPprotein kinase A - پروتئین کیناز Amitogen-activated protein kinase - پروتئین کیناز فعال با mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیExtracellular signal-regulated kinase (ERK) - کیناز تنظیم شده سیگنال خارج سلولی (ERK)glyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Extracellular signal-regulated kinases (ERKs) play important physiological roles in proliferation, differentiation and gene expression. ERK5 is twice the size of ERK1/2, the amino-terminal half contains the kinase domain that shares the homology with ERK1/2 and TEY activation motif, whereas the carboxy-terminal half is unique. In this study, we examined the cross-talk mechanism between G-protein-coupled receptors (GPCRs) and receptor tyrosine kinases, focusing on ERK1/2 and 5. The pretreatment of rat pheochromocytoma cells (PC12) with pertussis toxin (PTX) specifically enhanced epidermal growth factor (EGF)-induced ERK5 phosphorylation. In addition, lysophosphatidic acid (LPA) attenuated the EGF-induced ERK5 phosphorylation in LPA1 receptor- and Gi/o-dependent manners. On the other hand, LPA alone activated ERK1/2 via Gβγ subunits and Ras and potentiated EGF-induced ERK1/2 phosphorylation at late time points. These results suggest Gi/o negatively regulates ERK5, while it positively regulates ERK1/2. LPA did not affect cAMP levels after EGF treatment, and the reagents promoting cAMP production such as forskolin and cholera toxin also attenuated the EGF-induced ERK5 phosphorylation, indicating that the inhibitory effect of LPA on ERK5 inhibition via Gi/o is not due to inhibition of adenylyl cyclase by Gαi/o. However, the inhibitory effect of LPA on ERK5 was abolished in PC12 cells stably overexpressing C-terminus of GPCR kinase2 (GRK2), and overexpression of Gβ1 and γ2 subunits also suppressed ERK5 phosphorylation by EGF. In response to LPA, Gβγ subunits interacted with EGF receptor in a time-dependent manner. These results strongly suggest that LPA negatively regulates the EGF-induced ERK5 phosphorylation through Gβγ subunits.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 20, Issue 7, July 2008, Pages 1275-1283
Journal: Cellular Signalling - Volume 20, Issue 7, July 2008, Pages 1275-1283
نویسندگان
Yutaro Obara, Yumiko Okano, Sachiko Ono, Arata Yamauchi, Tomohiro Hoshino, Hitoshi Kurose, Norimichi Nakahata,