کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1977222 1539284 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Teratogenicity, genotoxicity and oxidative stress in zebrafish embryos (Danio rerio) co-exposed to arsenic and atrazine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Teratogenicity, genotoxicity and oxidative stress in zebrafish embryos (Danio rerio) co-exposed to arsenic and atrazine
چکیده انگلیسی

Arsenic and atrazine are common environmental contaminants probably due to their extensive use as pesticides on agricultural farmlands. In this study, zebrafish embryos were exposed to 0.8 mM arsenic, 0.1 mM atrazine or mixture of both for 96 h, and various indices which are indicative of teratogenicity (egg coagulation, growth retardation, edema formation, hatching success, scoliosis), genotoxicity (DNA tail moments) and oxidative stress (lipid peroxidation and reduced glutathione (GSH) levels, catalase and glutathione peroxidase activities) were determined. The negative control were exposed to 0.5% DMSO while the positive control group were exposed to 4 mg/L 3,4 dichloroaniline. Egg coagulation was highest in the positive control (85%), followed by the group that was exposed to mixture of arsenic and atrazine (30%) and least in the arsenic-exposed group (20%). The incidences of edema (59%) and growth retardation (35.2%) were more frequent in the group that was exposed to contaminant mixture and least in atrazine-exposed group where incidences of both edema and growth retardation were 15%. The incidence of scoliosis ranged between 20% in arsenic-exposed group and 10% in atrazine-exposed group. Hatching success was generally high in all the groups ranging between 95% in atrazine-exposed group and 88% in the group that was exposed to mixture of arsenic and atrazine. There was no evidence of teratogenic effect in the negative control group. DNA tail moments and lipid peroxidation levels increased significantly while GSH levels and catalase activity decreased significantly in contaminant-exposed groups, especially the mixture compared to the negative control. There was no significant change in GPx activity in the exposed groups compared to the negative control. The results of this study demonstrate that both arsenic and atrazine are potentially teratogenic and genotoxic, and can cause oxidative stress in zebrafish embryos, and these effects are potentiated by toxic interactions between the two contaminants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volumes 172–173, June–July 2015, Pages 7–12
نویسندگان
, , ,