Restricting the ligation step of non-homologous end-joining

Non-homologous end-joining is an important pathway for repairing DNA double-strand breaks. The budding yeast Saccharomyces cerevisiae possesses two proteins, Nej1/Lif2 and Ntr1/Spp382, which play a role in restricting the activity of Dnl4–Lif1, the complex that executes the final ligation step of this process.