کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1993360 1064657 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques
ترجمه فارسی عنوان
تصویربرداری ایزوتوپ پایدار از نمونه های بیولوژیکی با طیف سنجی جرم یونی متوسط ​​و تکنیک های مکمل
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

Stable isotopes are ideal labels for studying biological processes because they have little or no effect on the biochemical properties of target molecules. The NanoSIMS is a tool that can image the distribution of stable isotope labels with up to 50 nm spatial resolution and with good quantitation. This combination of features has enabled several groups to undertake significant experiments on biological problems in the last decade. Combining the NanoSIMS with other imaging techniques also enables us to obtain not only chemical information but also the structural information needed to understand biological processes. This article describes the methodologies that we have developed to correlate atomic force microscopy and backscattered electron imaging with NanoSIMS experiments to illustrate the imaging of stable isotopes at molecular, cellular, and tissue scales. Our studies make it possible to address 3 biological problems: (1) the interaction of antimicrobial peptides with membranes; (2) glutamine metabolism in cancer cells; and (3) lipoprotein interactions in different tissues.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 68, Issue 2, 1 July 2014, Pages 317–324
نویسندگان
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