کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2035235 | 1072152 | 2014 | 15 صفحه PDF | دانلود رایگان |
• Microtubule +TIP protein APC is an RNA-binding protein
• HITS-CLIP identifies 260 mRNA targets, which are highly related to APC functions
• APC target β2B-tubulin is axonally synthesized and localized to dynamic microtubules
• Tubulin mRNA regulation mediates growth cone expansion and neuron migration
SummaryAdenomatous polyposis coli (APC) is a microtubule plus-end scaffolding protein important in biology and disease. APC is implicated in RNA localization, although the mechanisms and functional significance remain unclear. We show APC is an RNA-binding protein and identify an RNA interactome by HITS-CLIP. Targets were highly enriched for APC-related functions, including microtubule organization, cell motility, cancer, and neurologic disease. Among the targets is β2B-tubulin, known to be required in human neuron and axon migration. We show β2B-tubulin is synthesized in axons and localizes preferentially to dynamic microtubules in the growth cone periphery. APC binds the β2B-tubulin 3′ UTR; experiments interfering with this interaction reduced β2B-tubulin mRNA axonal localization and expression, depleted dynamic microtubules and the growth cone periphery, and impaired neuron migration. These results identify APC as a platform binding functionally related protein and RNA networks, and suggest a self-organizing model for the microtubule to localize synthesis of its own subunits.
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Journal: - Volume 158, Issue 2, 17 July 2014, Pages 368–382