کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2035653 1072201 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probabilistic Splicing of Dscam1 Establishes Identity at the Level of Single Neurons
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Probabilistic Splicing of Dscam1 Establishes Identity at the Level of Single Neurons
چکیده انگلیسی


• Alternative splicing of Dscam1 is visualized in vivo using splicing reporters
• Cell-type- or spatial-specific splicing patterns between animals are not observed
• Alternative splicing of Dscam1 is probabilistic at the level of single neurons
• Splicing patterns suggest a widespread role for Dscam1 in self-avoidance

SummaryThe Drosophila Dscam1 gene encodes a vast number of cell recognition molecules through alternative splicing. These exhibit isoform-specific homophilic binding and regulate self-avoidance, the tendency of neurites from the same cell to repel one another. Genetic experiments indicate that different cells must express different isoforms. How this is achieved is unknown, as expression of alternative exons in vivo has not been shown. Here, we modified the endogenous Dscam1 locus to generate splicing reporters for all variants of exon 4. We demonstrate that splicing does not occur in a cell-type-specific fashion, that cells sharing the same anatomical location in different individuals express different exon 4 variants, and that the splicing pattern in a given neuron can change over time. We conclude that splicing is probabilistic. This is compatible with a widespread role in neural circuit assembly through self-avoidance and is incompatible with models in which specific isoforms of Dscam1 mediate homophilic recognition between processes of different cells.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 155, Issue 5, 21 November 2013, Pages 1166–1177
نویسندگان
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