کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2035808 | 1072225 | 2011 | 12 صفحه PDF | دانلود رایگان |
SummaryGlucose is catabolized in yeast via two fundamental routes, glycolysis and the oxidative pentose phosphate pathway, which produces NADPH and the essential nucleotide component ribose-5-phosphate. Here, we describe riboneogenesis, a thermodynamically driven pathway that converts glycolytic intermediates into ribose-5-phosphate without production of NADPH. Riboneogenesis begins with synthesis, by the combined action of transketolase and aldolase, of the seven-carbon bisphosphorylated sugar sedoheptulose-1,7-bisphosphate. In the pathway's committed step, sedoheptulose bisphosphate is hydrolyzed to sedoheptulose-7-phosphate by the enzyme sedoheptulose-1,7-bisphosphatase (SHB17), whose activity we identified based on metabolomic analysis of the corresponding knockout strain. The crystal structure of Shb17 in complex with sedoheptulose-1,7-bisphosphate reveals that the substrate binds in the closed furan form in the active site. Sedoheptulose-7-phosphate is ultimately converted by known enzymes of the nonoxidative pentose phosphate pathway to ribose-5-phosphate. Flux through SHB17 increases when ribose demand is high relative to demand for NADPH, including during ribosome biogenesis in metabolically synchronized yeast cells.
Graphical AbstractFigure optionsDownload high-quality image (164 K)Download as PowerPoint slideHighlights
► Riboneogenesis converts three-carbon glycolytic intermediates into ribose
► Sedoheptulose-1,7-bisphosphate is a key intermediate in riboneogenesis in yeast
► A sedoheptulose bisphosphatase, Shb17, thermodynamically drives ribose biosynthesis
► Flux through Shb17 is high when demand for ribose exceeds that for reducing power
Journal: - Volume 145, Issue 6, 10 June 2011, Pages 969–980