p38 MAPK is involved in human neutrophil chemotaxis induced by L-amino acid oxidase from Calloselasma rhodosthoma

• Cr-LAAO showed no toxicity on neutrophils.
• Cr-LAAO in its native form induced the neutrophil chemotaxis.
• p38MAPK and PI3K and have a role as signaling pathways of CR-LAAO induced chemotaxis.
• Cr-LAAO induced reactive oxygen species production and stimulated phagocytosis in neutrophils.
• Cr-LAAO stimulate neutrophils to release IL-6, IL-8, MPO, LTB4 and PGE2.

The action of LAAO, an L-amino acid oxidase isolated from Calloselasma rhodosthoma snake venom, on isolated human neutrophil function was investigated. Cr-LAAO showed no toxicity on neutrophils. Cr-LAAO in its native form induced the neutrophil chemotaxis, suggesting that its primary structure is essential for stimulation the cell. p38 MAPK and PI3K have a role as signaling pathways of CR-LAAO induced chemotaxis. This toxin also induced the production of hydrogen peroxide and stimulated phagocytosis in neutrophils. Furthermore, Cr-LAAO was able to stimulate neutrophils to release IL-6, IL-8, MPO, LTB4 and PGE2. Together, the data showed that the Cr-LAAO triggers relevant proinflammatory events.