کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2085874 1545479 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Automated Detection of Binucleated Cell and Micronuclei using CellProfiler 2.0 Software
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Automated Detection of Binucleated Cell and Micronuclei using CellProfiler 2.0 Software
چکیده انگلیسی

Micronucleus assay in human peripheral lymphocytes usually used to assess chromosomal damage. Manual scoring of micronuclei can be time consuming and large numbers of binucleated cells have to be analyzed to obtain statistically relevant data. Automation of the micronuclei analysis using image processing analysis software can provide a faster and more reliable analysis of micronucleus assay. Here the used of CellProfiler an open access cell image analysis software for automatic detection of binucleated cells and micronuclei were reported. We aimed to know whether there was a significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. Wilcoxon Rank test was used for statistical analysis to test H0 hypothesis that there was no significant difference in the number of binucleated cells and micronuclei that obtained by manual and CellProfiler counting. We analyzed 135 images for both manual and CellProfiler counting. Our results showed that there was no significant difference between manual and CellProfiler counting for binucleated cells (P = 0.851) and for micronuclei (P = 0.917). In conclusion, the binucleated cells and micronuclei counting using CellProfiler were comparable but not better than manual counting.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: HAYATI Journal of Biosciences - Volume 20, Issue 4, December 2013, Pages 151–156
نویسندگان
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