کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2096258 1082159 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polymerase subunit gamma 2 affects porcine oocyte maturation and subsequent embryonic development
ترجمه فارسی عنوان
گاما 2 زیرمجموعه پلیمریزاسیون بر بلوغ تخمک های گوسفند و رشد جنین بعد از آن تأثیر می گذارد
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

Deoxyribonucleic acid polymerase subunit gamma (POLG) is an enzyme encoded by the mitochondrial Polg gene. Polymerase (DNA directed), gamma 2, accessory subunit, also known as POLG2, is involved in mitochondrial replication. In the present study, we examined the role of Polg2 in the maturation of porcine oocytes. After Polg2 knockdown, the mitochondrial DNA copy number was significantly (P < 0.05) lower than that in the control group. However, there was no decrease in mitochondrial membrane potential. The decrease in mitochondrial DNA copy number led to reductions in adenosine-5′-triphosphate content (P < 0.05) and the maturation rate (P < 0.05) of oocytes. Furthermore, in the Polg2-knockdown group, maturation-promoting factor activity was decreased (P < 0.05) and the percentage of oocytes displaying abnormal actin filaments and microtubules was significantly increased (P < 0.05). This likely led to the reduced development rate and number of cells per blastocyst in this group (P < 0.05). In conclusion, Polg2 seems to be critical for mitochondrial replication and regulation of adenosine-5′-triphosphate content and affects porcine oocyte maturation and subsequent embryonic development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 83, Issue 1, 1 January 2015, Pages 121–130
نویسندگان
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